HSD3B1 Rabbit Polyclonal Antibody

About this product

bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta5-steroid precursors to 3-oxo-Delta4-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD+ as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity.

Specifications

Catalog number

HA500082

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

3 beta and steroid delta isomerase 1 antibody 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody 3 beta HSD I antibody 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I antibody 3-beta-HSD I antibody 3-beta-hydroxy-5-ene steroid dehydrogenase antibody 3BETAHSD antibody 3BH antibody 3BHS1_HUMAN antibody 3BHSD antibody Delta-5-3-ketosteroid isomerase antibody HGNC:5217 antibody HSD3B antibody HSD3B1 antibody HSDB3 antibody HSDB3A antibody Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1 antibody Hydroxy delta 5 steroid dehydrogenase antibody Progesterone reductase antibody SDR11E1 antibody Steroid Delta-isomerase antibody trophoblast antigen antibody Trophoblast antigen FDO161G antibody

Abbreviation

3 beta-hydroxysteroid dehydrogenase /Delta 5-->4-isomerase type 1 3-beta-HSD I 3-beta-hydroxy-5-ene steroid dehydrogenase 3-beta-hydroxy-Delta (5)-steroid dehydrogenase 3-beta-hydroxysteroid 3-dehydrogenase Delta-5-3-ketosteroid isomerase Dihydrotestosterone oxidoreductase Steroid Delta-isomerase Trophoblast antigen FDO161G1 P

Swiss Prot

P14060 Human

Cellular Locus

Endoplasmic reticulum, Membrane, Mitochondrion.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within human HSD3B1 aa 320-373.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

Human placenta tissue lysates, MCF-7, LOVO, human placenta tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000-1:5,000;IF-Cell: 1:50-1:200;IHC-P: 1:100-1:500

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

42 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No