AKT2 Rabbit Polyclonal Antibody

About this product

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. One of the few specific substrates of AKT2 identified recently is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake.

Specifications

Catalog number

HA500091

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Akt2 antibody AKT2_HUMAN antibody HIHGHH antibody Murine thymoma viral (v-akt) homolog 2 antibody murine thymoma viral (v-akt) homolog-2 antibody Oncogene AKT2 protein kinase B beta antibody PKB antibody PKB beta antibody PKBB antibody PKBBETA antibody PRKBB antibody Protein kinase Akt 2 antibody Protein kinase Akt-2 antibody Protein kinase B beta antibody RAC beta antibody rac protein kinase beta antibody RAC-BETA antibody RAC-beta serine/threonine-protein kinase antibody RAC-PK-beta antibody RACbeta antibody v akt murine thymoma viral oncogene homolog 2 antibody V-AKT murine thymoma viral oncogene homolog 2 antibody

Abbreviation

AKT2

Swiss Prot

P31751 Human, Q60823 Mouse, P47197 Rat

Cellular Locus

Nucleus, Cell membrane, Early endosome, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within C-terminal human AKT2.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P

Positive Control

A549 cell lysate, Hela cell lysate, A431 cell lysate, 293 cell lysate, rat brain tissue lysate, mouse pancreas tissue lysate, rat brain tissue, rat kidney tissue, mouse testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:100-1:500

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 56 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No