FLI1 Rabbit Polyclonal Antibody

About this product

Fli-1 is a member of the ETS transcription factor family that was first identified in erythroleukemias induced by Friend Murine Leukemia Virus (F-MuLV). Fli-1 is activated through retroviral insertional mutagenesis in 90% of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event (s) associated with terminal differentiation to proliferation. In addition to Friend erythroleukemia, proviral integration at the fli-1 locus also occurs in leukemias induced by the 10A1, Graffi, and Cas-Br-E viruses. Fli-1 aberrant expression is also associated with chromosomal abnormalities in humans. In pediatric Ewing’s sarcoma a chromosomal translocation generates a fusion of the 5’ transactivation domain of EWSR1 (also known as EWS) with the 3’ Ets domain of Fli-1. The resulting fusion oncoprotein, EWS/Fli-1, acts as an aberrant transcriptional activator. with strong transforming capabilities. A recent study has demonstrated high levels of Fli-1 expression in several benign and malignant neoplasms using immunohistochemistry.

Specifications

Catalog number

HA500149

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ERGB transcription factor antibody Ewing Sarcoma breakpoint region 2 antibody EWSR 2 antibody EWSR2 antibody FLI 1 antibody FLI 1 proto oncogene antibody Fli-1 proto-oncogene, ETS transcription factor antibody FLI1 antibody FLI1 EWS fusion gene antibody FLI1 proto oncogene antibody FLI1_HUMAN antibody Friend leukemia integration 1 (FLI1) transcription factor antibody Friend leukemia integration 1 transcription factor antibody Friend leukemia virus integration 1 antibody Proto-oncogene Fli-1 antibody Sarcoma breakpoint region 2 (EWSR2) antibody SIC 1 antibody SIC1 antibody Transcription factor ERGB antibody Viral integration region FLI1 antibody Viral integration region FLI1, mouse, homolog of antibody

Abbreviation

FLI1

Swiss Prot

Q01543 Human, P26323 Mouse

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human FLI1 aa 1-200.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

IHC-P, WB

Positive Control

NIH/3T3 cell lysate, Jurkat cell lysate, rat spleen tissue, human colon tissue, human spleen tissue, mouse spleen tissue.

Concentration

1 mg/mL

Dilution

IHC-P: 1:100-1:500 ;WB: 1:500-1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

51 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No