CDKN1A/P21 Rabbit Polyclonal Antibody

About this product

p21 is a potent cyclin-dependent kinase inhibitor (CKI). The p21 (CIP1/WAF1) protein binds to and inhibits the activity of cyclin-CDK2, -CDK1, and -CDK4/6 complexes, and thus functions as a regulator of cell cycle progression at G1 and S phase. p21 interacts with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. However p21 may inhibit apoptosis and does not induce cell death on its own. The ability of p21 to inhibit apoptosis in response to replication fork stress has also been reported. Studies of p53 dependent cell cycle arrest in response to DNA damage identified p21 as the primary mediator of downstream cell cycle arrest. p21 is negatively regulated by ubiquitin ligases both over the course of the cell cycle and in response to DNA damage. Cytoplasmic p21 expression can be significantly correlated with lymph node metastasis, distant metastases, advanced TNM stage, depth of invasion and OS.

Specifications

Catalog number

HA500156

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

CAP20 antibody CDK-interacting protein 1 antibody CDKI antibody CDKN1 antibody Cdkn1a antibody CDN1A_HUMAN antibody CIP1 antibody Cyclin Dependent Kinase Inhibitor 1A antibody Cyclin-dependent kinase inhibitor 1 antibody Cyclin-dependent kinase inhibitor 1A (P21) antibody Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody DNA Synthesis Inhibitor antibody MDA-6 antibody MDA6 antibody Melanoma differentiation-associated protein 6 antibody Melanoma differentiation-associated protein antibody p21 antibody P21 protein antibody p21CIP1 antibody p21Cip1/Waf1 antibody p21WAF antibody PIC1 antibody SDI1 antibody SLC12A9 antibody WAF1 antibody Wild type p53 activated fragment 1 (WAF1) antibody Wild type p53 activated fragment 1 antibody Wildtype p53-activated fragment 1 antibody

Abbreviation

Cyclin-dependent kinase inhibitor 1 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 MDA-6 p21

Swiss Prot

P38936 Human, P39689 Mouse

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within human CDKN1A/P21 aa 1-164.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P

Positive Control

MCF7 treated with 10μM NUTLIN 3A for 24 hours cell lysate, U-2 OS treated with 10μM NUTLIN 3A for 24 hours cell lysate, mouse colon tissue lysates, human esophagus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 18 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No