DDX4 Rabbit Polyclonal Antibody

About this product

The Vasa gene is a member of the DEAD box family of RNA helicases in Drosophila melanogaster. Its human ortholog, Ddx4, is located on human chromosome 5q. It is syntenic to mouse chromosome 13, where the mouse vasa gene is located. The gene is conserved in many invertebrates and vertebrate species such as Caenorhabditis elegans, Xenopus, Zebrafish, flatworms, echinoderms, molluscs, nematodes, mice and rats as an important part of germ line maintenance and function. One of main function of Vasa protein is in germ cell determination and function. It uses ATP dependent RNA helicase catalytic activity to regulate the translation of multiple mRNAs. Vasa unwinds the duplex RNA by binding and bending short stretches of the duplex in a non-processive manner. The conserved domain may act as chaperones by unwinding RNA secondary structures and refolding properly. pre-mRNA splicing, ribosome biogenesis, nuclear export, translational regulation and degradation. Vasa was found to bind RNA in a sequence-specific manner. In the Drosophila embryos, Vasa binds the Uracil rich motif of the mei-P26 UTR. A mutation in Vasa reduced the interaction of between Mei-P26 and initiation factor elF58 which in turn significantly reduced translation of the gene. Recent evidence in invertebrates suggests that Vasa has a role in multipotent stem cells, but the exact function is unknown.

Specifications

Catalog number

HA500161

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

DDX 4 antibody Ddx4 antibody DDX4_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody DEAD box protein 4 antibody DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody MVH antibody Probable ATP dependent RNA helicase DDX4 antibody Probable ATP-dependent RNA helicase DDX4 antibody VASA antibody Vasa homolog antibody

Abbreviation

Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA

Swiss Prot

Q9NQI0 Human, Q61496 Mouse, Q64060 Rat

Cellular Locus

Cytoplasm, perinuclear region.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human DDX4 aa 1-200.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-Fr, IHC-P

Positive Control

Mouse testis tissue, mouse testis tissue lysate, rat testis tissue lysate, human testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-Fr: 1:200 ;IHC-P: 1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 79 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No