PLK1 Rabbit Polyclonal Antibody

About this product

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. PLK1 is high on non-attached kinetochores suggesting a role of PLK1 in kinetochore attachment or in spindle assembly checkpoint (SAC) regulation. Required for kinetochore localization of BUB1B. Regulates the dissociation of cohesin from chromosomes by phosphorylating cohesin subunits such as STAG2/SA2.

Specifications

Catalog number

HA500281

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Cell cycle regulated protein kinase antibody PLK 1 antibody PLK antibody PLK-1 antibody plk1 antibody PLK1_HUMAN antibody Polo like kinase 1 antibody Polo-like kinase 1 antibody Serine/threonine protein kinase 13 antibody Serine/threonine protein kinase PLK1 antibody Serine/threonine-protein kinase 13 antibody Serine/threonine-protein kinase PLK1 antibody STPK 13 antibody STPK13 antibody

Abbreviation

Serine/threonine-protein kinase PLK1 Polo-like kinase 1 PLK-1 Serine/threonine-protein kinase 13 STPK13

Swiss Prot

P53350 Human, Q07832 Mouse

Cellular Locus

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within human PLK1 aa 1-50 / 603.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, FC

Positive Control

HeLa whole cell lysate, HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate, HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate, HT-29 whole cell lysate, HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate, HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate, HepG2 cell lysate, Hela cell lysate, mouse testis tissue lysate, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;FC: 1:500-1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 68 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No