AP2B1 Rabbit Polyclonal Antibody

About this product

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis.. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10.. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.

Specifications

Catalog number

HA500315

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Adapter related protein complex 2 beta 1 subunit antibody Adapter-related protein complex 2 beta subunit antibody Adaptin, beta 2 (beta) antibody Adaptor protein complex AP-2 subunit beta antibody Adaptor related protein complex 2, beta 1 subunit antibody ADTB2 antibody AP-2 complex subunit beta antibody AP105B antibody AP2 BETA antibody Ap2b1 antibody AP2B1_HUMAN antibody Beta adaptin antibody Beta-2-adaptin antibody Beta-adaptin antibody Beta2 adaptin antibody CLAPB1 antibody Clathrin assembly protein complex 2 beta large chain antibody Clathrin associated/assembly/adaptor protein, large, beta 1 antibody DKFZp781K0743 antibody Plasma membrane adaptor HA2/AP2 adaptin beta subunit antibody

Abbreviation

AP-2 complex subunit beta AP105B Adaptor protein complex AP-2 subunit beta Adaptor-related protein complex 2 subunit beta Beta-2-adaptin Beta-adaptin Clathrin assembly protein complex 2 beta large chain Plasma membrane adaptor HA2/AP2 adaptin beta subuni

Swiss Prot

P63010 Human, Q9DBG3 Mouse, P62944 Rat

Cellular Locus

Cell membrane, Coated pit, Membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human AP2B1 aa 400-600 / 937.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

A549 cell lysate, PC-3M cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat kidney tissue, human liver tissue, A549.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:100 ;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 105 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No