YTHDF2 Rabbit Polyclonal Antibody

About this product

Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development. Also required during spermatogenesis: regulates spermagonial adhesion by promoting degradation of m6A-containing transcripts coding for matrix metallopeptidases. Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation. Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets.

Specifications

Catalog number

HA500351

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

9430020E02Rik antibody CAHL antibody CLL associated antigen KW 14 antibody CLL-associated antigen KW-14 antibody HGRG8 antibody High glucose regulated protein 8 antibody High-glucose-regulated protein 8 antibody NY REN 2 antibody Renal carcinoma antigen NY REN 2 antibody Renal carcinoma antigen NY-REN-2 antibody YTH domain family 2 antibody YTH domain family member 2 antibody YTH domain family protein 2 antibody YTH N6 methyladenosine RNA binding protein 2 antibody YTHD2_HUMAN antibody YTHDF 2 antibody YTHDF2 antibody

Abbreviation

DF2 HGRG8 YTHDF2

Swiss Prot

Q9Y5A9 Human, Q91YT7 Mouse

Cellular Locus

Nucleus, cytosol.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Full length of Human YTHDF2.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell

Positive Control

293T cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, NIH/3T3.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 62 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No