PPP3CB Rabbit Polyclonal Antibody

About this product

Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca2+-mediated signals. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32. Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB (By similarity). May play a role in skeletal muscle fiber type specification (By similarity). Unlike for protein substrates, PPP3CB activity towards synthetic phosphatase substrate p-nitrophenyl phosphate (pNPP) is increased in presence of the immunosuppressant complex FKBP12-FK506. Activated by Ca2+-bound calmodulin following an increase in intracellular Ca2+. At low Ca2+ concentrations, the catalytic subunit (also known as calcineurin A) is inactive and is bound to the regulatory subunit (also known as calcineurin B) in which only two high-affinity binding sites are occupied by Ca2+. In response to elevated calcium levels, the occupancy of the low-affinity sites on calcineurin B by Ca2+ causes a conformational change of the C-terminal regulatory domain of calcineurin A, resulting in the exposure of the calmodulin-binding domain and in the partial activation of calcineurin A. The subsequent binding of Ca2+-bound calmodulin leads to the displacement of the autoinhibitory domain from the active site and possibly of the autoinhibitory segment from the substrate binding site which fully activates calcineurin A.

Specifications

Catalog number

HA500370

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Calcineurin A beta antibody Calcineurin A2 antibody Calcineurin B, formerly antibody Calmodulin dependent calcineurin A subunit beta isoform antibody Calmodulin-dependent calcineurin A subunit beta isoform antibody CALNA 2 antibody CALNA2 antibody CALNB antibody CAM PRP catalytic subunit antibody CAM-PRP catalytic subunit antibody CNA2 antibody CnAbeta antibody PP2BB_HUMAN antibody PP2Bbeta antibody PPP3CB antibody Protein phosphatase 2B, catalytic subunit, beta isoform, formerly antibody Protein phosphatase 3 (formerly 2B) catalytic subunit beta isoform antibody Protein phosphatase 3 catalytic subunit beta isoform antibody Protein phosphatase 3 catalytic subunit beta isozyme antibody Serine/threonine protein phosphatase 2B catalytic subunit beta isoform antibody Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform antibody

Abbreviation

Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (EC:3.1.3.162 Publications) CAM-PRP catalytic subunit Calmodulin-dependent calcineurin A subunit beta isoform CNA beta PPP3CB CALNA2 CALNB CNA2

Swiss Prot

P16298 Human, P48453 Mouse, P20651 Rat

Cellular Locus

Cytoplasm

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human PPP3CB aa 475-524.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P

Positive Control

Human brain tissue lysate, rat brain tissue lysate, rat brain tissue, mouse skeletal muscle tissue.

Concentration

1.8 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:600

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 59 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No