Histone H2B Rabbit Polyclonal Antibody

About this product

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.

Specifications

Catalog number

HA500381

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

GL105 antibody H2B GL105 antibody H2B histone family member O antibody H2B histone family member S antibody H2B.1 antibody H2B.1 B antibody H2B.b antibody H2B.c antibody H2B.d antibody H2B.e antibody H2B.f antibody H2B.j antibody H2B.q antibody H2B/b antibody H2B/c antibody H2B/d antibody H2B/e antibody H2B/f antibody H2B/j antibody H2B/o antibody H2B/q antibody H2BFB antibody H2BFC antibody H2BFD antibody H2BFE antibody H2BFF antibody H2BFJ antibody H2BFO antibody H2BFQ antibody H2BFS antibody H2BGL105 antibody H2BQ antibody HIRIP2 antibody HIST1H2BB antibody HIST1H2BD antibody HIST1H2BH antibody HIST1H2BL antibody HIST1H2BM antibody HIST1H2BN antibody HIST2H2BE antibody histone cluster 2 antibody Histone H2B antibody histone H2B GL105 antibody Histone H2B type 1 B antibody Histone H2B type 1 D antibody Histone H2B type 1 H antibody Histone H2B type 1 L antibody Histone H2B type 1 M antibody Histone H2B type 1 N antibody Histone H2B type 2 E antibody Histone H2B.q antibody histone protein antibody

Swiss Prot

O60814 Human, P06899 Human, P23527 Human, P33778 Human, P57053 Human, P58876 Human, P62807 Human, Q16778 Human, Q5QNW6 Human, Q8N257 Human, Q93079 Human, Q99877 Human, Q99879 Human, Q99880 Human, P10854 Mouse, Q64475 Mouse, Q00729 Rat

Cellular Locus

Nucleus

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within C-terminal human Histone H2B.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P

Positive Control

MCF-7 cell lysate, Hela cell lysate, A549 cell lysate, 293T cell lysate, THP-1 cell lysate, K562 cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, zebrafish tissue lysate, PC-12 cell lysate, RAW264.7 cell lysate, rat large intestine tissue, rat liver tissue, human breast tissue.

Concentration

1.7 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:5,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 14 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No