NF-kappaB p65 Rabbit Polyclonal Antibody

About this product

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cell.

Specifications

Catalog number

HA500402

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody MGC131774 antibody NF kappa B p65delta3 antibody nfkappabp65 antibody NFkB p65 antibody NFKB3 antibody Nuclear factor kappaB antibody Nuclear Factor NF Kappa B p65 Subunit antibody Nuclear factor NF-kappa-B p65 subunit antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody OTTHUMP00000233473 antibody OTTHUMP00000233474 antibody OTTHUMP00000233475 antibody OTTHUMP00000233476 antibody OTTHUMP00000233900 antibody p65 antibody p65 NF kappaB antibody p65 NFkB antibody relA antibody TF65_HUMAN antibody Transcription factor NFKB3 antibody Transcription factor p65 antibody v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody V rel avian reticuloendotheliosis viral oncogene homolog A antibody v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody NF-κB p65 NF κB p65 NF-κB p65

Abbreviation

Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 RELA NFKB3

Swiss Prot

Q04206 Human, Q04207 Mouse,

Cellular Locus

Nucleus, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human NF-κB p65 aa 450-550.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

NIH/3T3 cell lysate, Hela cell lysate, MCF-7 cell lysate, HepG2 cell lysate, 293 cell lysate, human breast tissue, SH-SY5Y, PC-12.

Concentration

1.98 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200 ;IF-Cell: 1:200

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 65 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No