Phospho-ATR (T1989) Rabbit Polyclonal Antibody

About this product

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling. Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Specifications

Catalog number

HA500405

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Ataxia telangiectasia and Rad3 related antibody Ataxia telangiectasia and Rad3-related protein antibody ATR antibody ATR_HUMAN antibody FCTCS antibody FRAP Related Protein 1 antibody FRAP-related protein 1 antibody FRP1 antibody MEC1 antibody MEC1 mitosis entry checkpoint 1 homolog antibody Protein kinase ATR antibody RAC3 antibody Rad3 related protein antibody SCKL antibody SCKL1 antibody Serine/threonine protein kinase ATR antibody Serine/threonine-protein kinase ATR antibody

Abbreviation

Serine/threonine-protein kinase ATR Ataxia telangiectasia and Rad3-related protein FRAP-related protein 1 ATR FRP1

Swiss Prot

Q13535 Human

Cellular Locus

Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide containing pThr1989 site Peptides for Immunizaiton 1984-1995aa.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB

Positive Control

Hela cell lysate, HeLa treated with 1.5mM hydroxyurea for 16 hours cell lysate.

Concentration

2.13 mg/mL

Dilution

WB: 1: 1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 301 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No