TNNC1 Rabbit Polyclonal Antibody

About this product

Troponin C, also known as TN-C or TnC, is a protein that resides in the troponin complex on actin thin filaments of striated muscle (cardiac, fast-twitch skeletal, or slow-twitch skeletal) and is responsible for binding calcium to activate muscle contraction. Troponin C is encoded by the TNNC1 gene in humans for both cardiac and slow skeletal muscle. The structural domain of cTnC (cCTnC) is anchored to troponin I and T, forming the so-called IT arm, made up of cTnC93-161, cTnI41-135 and cTnT235-286 (in the cardiac complex). cCTnC binds to helical cTnI41-60 via its large hydrophobic patch, stabilizing the Ca2+-bound open conformation of cCTnC and enhancing its affinity for Ca2+ (from Kd = 40 nM to Kd = 3 nM). cTnT235-286 forms a helical coiled coil with cTnI88-135 that binds to the opposite face of cCTnC. The IT arm is anchored to tropomyosin via adjacent segments of cTnT, so it is believed to move as a unit along with tropomyosin throughout the cardiac cycle. In the low calcium environment present during diastole (~100 nM), tropomyosin is anchored into the "blocked" position along the actin thin filament through the binding of the troponin I inhibitory (cTnI128-147) and C-terminal (cTnI160-209) regions. This prevents actin-myosin cross-bridging and effectively shuts off muscle contraction.

Specifications

Catalog number

HA500407

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Cardiac troponin C antibody CMD1Z antibody CMH13 antibody slow skeletal and cardiac muscles antibody Slow twitch skeletal/cardiac muscle troponin C antibody TN-C antibody TNC antibody TNNC antibody Tnnc1 antibody TNNC1 troponin C type 1 (slow) antibody TNNC1_HUMAN antibody Troponin C antibody troponin C type 1 (slow) antibody Troponin C, slow skeletal and cardiac muscles antibody Troponin C1, slow antibody

Abbreviation

TNNC1

Swiss Prot

P63316 Human, P19123 Mouse, P23693 Rat

Cellular Locus

Cardiac Troponin complex, troponin complex, cytosol.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length protein of human TNNC1.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Human heart tissue lysates, MG-63, SKOV-3, SW620, rat skeletal muscle tissue, rat heart tissue, mouse heart tissue.

Concentration

1 mg/mL

Dilution

WB:1:200-1:500;IF-Cell:1:50-1:100;IHC-P:1:200;FC:1:50

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

18 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No