PSMA4 Rabbit Polyclonal Antibody

About this product

Crystal structures of isolated 20S proteasome complex demonstrate that the two rings of beta subunits form a proteolytic chamber and maintain all their active sites of proteolysis within the chamber.[9] Concomitantly, the rings of alpha subunits form the entrance for substrates entering the proteolytic chamber. In an inactivated 20S proteasome complex, the gate into the internal proteolytic chamber are guarded by the N-terminal tails of specific alpha-subunit. The proteolytic capacity of 20S core particle (CP) can be activated when CP associates with one or two regulatory particles (RP) on one or both side of alpha rings. These regulatory particles include 19S proteasome complexes, 11S proteasome complex, etc. Following the CP-RP association, the confirmation of certain alpha subunits will change and consequently cause the opening of substrate entrance gate. Besides RPs, the 20S proteasomes can also be effectively activated by other mild chemical treatments, such as exposure to low levels of sodium dodecylsulfate (SDS) or NP-14. The eukaryotic proteasome recognized degradable proteins, including damaged proteins for protein quality control purpose or key regulatory protein components for dynamic biological processes. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. As a component of alpha ring, proteasome subunit alpha type-4 contributes to the formation of heptameric alpha rings and substrate entrance gate. Importantly, this subunit plays an critical role in the assembly of 19S base and 20S.

Specifications

Catalog number

HA500452

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

HC9 antibody HsT17706 antibody Macropain subunit C9 antibody MGC111191 antibody MGC12467 antibody MGC24813 antibody Multicatalytic endopeptidase complex subunit C9 antibody Proteasome (prosome macropain) subunit alpha type 4 antibody Proteasome alpha 4 subunit antibody Proteasome component C9 antibody Proteasome subunit alpha type 4 antibody Proteasome subunit alpha type-4 antibody Proteasome subunit HC9 antibody Proteasome subunit L antibody PSA4_HUMAN antibody PSC9 antibody PSMA 4 antibody psmA4 antibody

Abbreviation

PSMA4

Swiss Prot

P25789 Human, Q9R1P0 Mouse, P21670 Rat

Cellular Locus

Cytoplasm. Nucleus. Cytoplasm, P-body.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human PSMA4 aa 62-261.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P

Positive Control

Jurkat cell lysate, Hela cell lysate, K562 cell lysate, U937 cell lysate, rat testis tissue lysate, mouse cerebellum tissue lysate, human placenta tissue lysate, rat kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:800

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 29 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No