AP2A2 Rabbit Polyclonal Antibody

About this product

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10.

Specifications

Catalog number

HA500477

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

100 kDa coated vesicle protein C antibody Adaptor protein complex AP-2 subunit alpha-2 antibody Adaptor-related protein complex 2 subunit alpha-2 antibody Alpha-adaptin C antibody Alpha2-adaptin antibody AP-2 complex subunit alpha-2 antibody Ap2a2 antibody AP2A2_HUMAN antibody Clathrin assembly protein complex 2 alpha-C large chain antibody HIP-9 antibody Huntingtin yeast partner J antibody Huntingtin-interacting protein 9 antibody Huntingtin-interacting protein J antibody Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody

Abbreviation

AP2A2

Swiss Prot

O94973 Human, P17427 Mouse, P18484 Rat

Cellular Locus

Cell membrane, coated pit.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human AP2A2 aa 740-939 / 939.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P

Positive Control

Hela cell lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, human kidney tissue, mouse cerebellum tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:5,000 ;IHC-P: 1:200-1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

104 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No