p16INK4a Mouse Monoclonal Antibody [A4A3]

About this product

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Specifications

Catalog number

HA600027

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ARF antibody CDK4 inhibitor p16 INK4 antibody CDK4I antibody CDKN2 antibody CDKN2A antibody Cell cycle negative regulator beta antibody CMM2 antibody Cyclin dependent kinase 4 inhibitor A antibody Cyclin dependent kinase inhibitor 2A antibody INK4 antibody INK4a antibody Melanoma p16 inhibits CDK4 antibody MLM antibody MTS 1 antibody MTS1 antibody Multiple tumor suppressor 1 antibody p16 antibody p16 INK4a antibody p16INK4a antibody p19 antibody P19ARF antibody TP16 antibody

Abbreviation

Cyclin-dependent kinase inhibitor 2 Cyclin-dependent kinase 4 inhibitor A CDK4I Multiple tumor suppressor 1 MTS-1 p16-INK4a p16-INK4 p16INK4A

Swiss Prot

P42771 Human

Cellular Locus

Cytoplasm, Nucleus.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within human p16INK4a aa 1-156.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P

Positive Control

Hela cell lysates, human ovarian serous carcinoma tissue, human cervical poorly differentiated adenocarcinoma tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500 ;IHC-P: 1:100-1:500

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

16 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No