Histone H3 (acetyl K27) Mouse Monoclonal Antibody [A6D6]

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.

Specifications

Catalog number

HA600048

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

HIST1 cluster, H3J antibody Histone gene cluster 1, H3 histone family, member E antibody Histone gene cluster 1, H3G antibody H3 histone family, member J antibody HIST1 cluster, H3E antibody HIST1 cluster, H3I antibody Histone gene cluster 1, H3C antibody FLJ92264 antibody H3 histone family, member A antibody H3 histone family, member B antibody H3 histone family, member C antibody H3 histone family, member D antibody H3 histone family, member F antibody H3 histone family, member H antibody H3 histone family, member I antibody H3 histone family, member K antibody H3 histone family, member L antibody H3 histone family, member T antibody H3 histone, family 3A antibody H3.1 antibody H3.3A antibody H3/a antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/l antibody H3/t antibody H31_HUMAN antibody H3F1K antibody H3F3 antibody H3F3A antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FF antibody H3FH antibody H3FI antibody H3FJ antibody H3FK antibody H3FL antibody HIST1 cluster, H3A antibody HIST1 cluster, H3B antibody HIST1 cluster, H3C antibody HIST1 cluster, H3D antibody HIST1 cluster, H3F antibody HIST1 cluster, H3G antibody HIST1 cluster, H3H antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1, H3a antibody Histone 1, H3b antibody Histone 1, H3c antibody Histone 1, H3d antibody Histone 1, H3e antibody Histone 1, H3f antibody Histone 1, H3g antibody Histone 1, H3h antibody Histone 1, H3i antibody Histone 1, H3j antibody histone 3, H3 antibody histone cluster 1 H3 family member a antibody histone cluster 1 H3 family member b antibody histone cluster 1 H3 family member c antibody histone cluster 1 H3 family member d antibody histone cluster 1 H3 family member e antibody histone cluster 1 H3 family member f antibody histone cluster 1 H3 family member g antibody histone cluster 1 H3 family member h antibody histone cluster 1 H3 family member i antibody histone cluster 1 H3 family member j antibody Histone cluster 1, H3a antibody Histone cluster 1, H3b antibody Histone cluster 1, H3c antibody Histone cluster 1, H3d antibody Histone cluster 1, H3e antibody Histone cluster 1, H3f antibody Histone cluster 1, H3g antibody Histone cluster 1, H3i antibody Histone cluster 1, H3j antibody Histone gene cluster 1, H3 histone family, member A antibody Histone gene cluster 1, H3 histone family, member B antibody Histone gene cluster 1, H3 histone family, member C antibody Histone gene cluster 1, H3 histone family, member D antibody Histone gene cluster 1, H3 histone family, member F antibody Histone gene cluster 1, H3 histone family, member G antibody Histone gene cluster 1, H3 histone family, member H antibody Histone gene cluster 1, H3 histone family, member I antibody Histone gene cluster 1, H3 histone family, member J antibody Histone gene cluster 1, H3A antibody Histone gene cluster 1, H3B antibody Histone gene cluster 1, H3D antibody Histone gene cluster 1, H3E antibody Histone gene cluster 1, H3F antibody Histone gene cluster 1, H3H antibody Histone gene cluster 1, H3I antibody Histone gene cluster 1, H3J antibody Histone H 3 antibody Histone H3.1 antibody histone H3.1t antibody Histone H3.2 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody Histone H3/o antibody H3K27ac antibody

Abbreviation

Histone H3 H3K27ac

Swiss Prot

P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P68433 Mouse, Q6LED0 Rat

Cellular Locus

Nucleus, Chromosome.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human Histone H3 aa 20-40 (acetyl K27).

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, ChIP

Positive Control

PC-12 cell lysate, 293 cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, HeLa cell lysates, HeLa treated with Trichostatin A whole cell lysates, HUVEC, HeLa, human tonsil tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:600 ;FC: 1:500 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Purity

Protein G affinity purified.

Form

Liquid

Buffer

PBS (pH 7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No