eIF-6 Mouse Monoclonal Antibody [A6A3]

About this product

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis.

Specifications

Catalog number

HA600051

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

b (2)gcn antibody B (2)GCN homolog antibody B4 integrin interactor antibody Binding protein of beta-4 integrin antibody CAB antibody eIF-6 antibody EIF3A antibody EIF6 antibody Eukaryotic translation initiation factor 3A antibody Eukaryotic translation initiation factor 6 antibody IF6_HUMAN antibody Integrin beta 4 binding protein antibody ITGB4BP antibody OK/SW-cl.27 antibody p27 beta 4 integrin binding protein antibody p27 (BBP) antibody p27BBP antibody RP4-614O4.1 antibody

Abbreviation

EIF6

Swiss Prot

P56537 Human, O55135 Mouse, Q3KRD8 Rat

Cellular Locus

Cytoplasm, Nucleus, nucleolus.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human eIF-6 aa 1-200.

Isotype

IgG2a

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC

Positive Control

HeLa cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat kidney tissue lysate, rat testis tissue lysate, human colon cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, HepG2, NIH/3T3.

Concentration

2 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Purity

Protein G affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 27 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No