Bub1 Mouse Monoclonal Antibody [A6E10]

About this product

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Specifications

Catalog number

HA600053

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Bub1 antibody BUB1 budding uninhibited by benzimidazoles 1 homolog antibody BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody BUB1 mitotic checkpoint serine/threonine kinase antibody BUB1, S. cerevisiae, homolog of antibody BUB1_HUMAN antibody BUB1A antibody BUB1L antibody Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody Budding uninhibited by benzimidazoles 1 homolog antibody Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody hBUB1 antibody Homolog of mitotic checkpoint gene BUB1 antibody Mitotic checkpoint gene BUB1 antibody Mitotic checkpoint serine/threonine protein kinase BUB1 antibody Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody Mitotic spindle checkpoint kinase antibody Putative serine/threonine protein kinase antibody

Abbreviation

BUB1

Swiss Prot

O43683 Human

Cellular Locus

Nucleus, kinetochore.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within human Bub1 aa 1-200.

Isotype

IgG2a

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IP

Positive Control

Hela cell lysate, K562 cell lysate, 293T cell lysate.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IP: Use at an assay dependent concentration.

Purity

Protein G affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

122 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No