POLR2A Mouse Monoclonal Antibody [A5F4]

About this product

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines. Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD. Methylation and dimethylation have a repressive effect on target genes expression.

Specifications

Catalog number

HA600061

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

DNA directed RNA polymerase II A antibody DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibody DNA-directed RNA polymerase II subunit A antibody DNA-directed RNA polymerase II subunit RPB1 antibody DNA-directed RNA polymerase III largest subunit antibody hRPB220 antibody hsRPB1 antibody POLR2 antibody Polr2a antibody POLRA antibody Polymerase (RNA) II (DNA directed) polypeptide A 220kDa antibody Polymerase (RNA) II (DNA directed) polypeptide A antibody RNA polymerase II subunit B1 antibody RNA-directed RNA polymerase II subunit RPB1 antibody RPB1 antibody RPB1_HUMAN antibody RPBh1 antibody RpIILS antibody RPO2 antibody RPOL2 antibody

Abbreviation

POLR2A

Swiss Prot

P24928 Human, P08775 Mouse, D4A5A6 Rat

Cellular Locus

Cytoplasm, Nucleus, Chromosome.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human POLR2A aa 751-950 / 1,970.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC, ChIP

Positive Control

MCF-7 cell lysates, mouse lung tissue lysates, rat bladder tissue, SH-SY5Y.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500 ;FC: 1:100-1:500 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

217 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No