CENPB Mouse Monoclonal Antibody [A5F6]

About this product

This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Specifications

Catalog number

HA600071

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

CENP B antibody CENP-B antibody CENPB antibody CENPB_HUMAN antibody centromere autoantigen B antibody Centromere protein B antibody Centromere protein B, 80kDa antibody Major centromere autoantigen B antibody

Abbreviation

CENPB

Swiss Prot

P07199 Human, P27790 Mouse,

Cellular Locus

Nucleus, centromere.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human CENPB aa 1-200 / 599.

Isotype

IgG2a

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

A431 cell lysate, Daudi cell lysate, 3T3-L1 cell lysate, PC-12 cell lysate, SH-SY5Y, human kidney tissue, human skin tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:100-1:500

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 65 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No