Phospho-mTOR (S2448) Mouse Monoclonal Antibody [A5D5]

About this product

The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V (D)J recombination. FRAP can autophosphorylate on serine and bind to Rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression.

Specifications

Catalog number

HA600094

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody FK506 binding protein 12 rapamycin associated protein 1 antibody FK506 binding protein 12 rapamycin associated protein 2 antibody FK506 binding protein 12 rapamycin complex associated protein 1 antibody FK506-binding protein 12-rapamycin complex-associated protein 1 antibody FKBP rapamycin associated protein antibody FKBP12 rapamycin complex associated protein antibody FKBP12-rapamycin complex-associated protein 1 antibody FKBP12-rapamycin complex-associated protein antibody FLJ44809 antibody FRAP antibody FRAP1 antibody FRAP2 antibody Mammalian target of rapamycin antibody Mechanistic target of rapamycin antibody mTOR antibody MTOR_HUMAN antibody OTTHUMP00000001983 antibody RAFT1 antibody Rapamycin and FKBP12 target 1 antibody Rapamycin associated protein FRAP2 antibody Rapamycin target protein 1 antibody Rapamycin target protein antibody RAPT1 antibody Serine/threonine-protein kinase mTOR antibody

Abbreviation

Serine/threonine-protein kinase mTOR FK506-binding protein 12-rapamycin complex-associated protein 1 FKBP12-rapamycin complex-associated protein Mammalian target of rapamycin mTOR Mechanistic target of rapamycin Rapamycin and FKBP12 target 1 Rapamycin target protein 1

Swiss Prot

P42345 Human, Q9JLN9 Mouse

Cellular Locus

Lysosome, Mitochondrion outer membrane, Endoplasmic reticulum membrane, Cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human mTOR aa 2430-2480.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P

Positive Control

HeLa cell lysate, HeLa starved overnight then treated with 200nM PMA for 4 hours cell lysate, NIH/3T3 cell lysate treated with serum-starvation overnight, NIH/3T3 cell lysate treated with Insulin (100nM) for 10 minutes after serum-starvation overnight, human liver tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200

Purity

Protein G affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 289 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No