IL-1 beta Mouse Monoclonal Antibody [A7F9]

About this product

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Specifications

Catalog number

HA601002

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Catabolin antibody H1 antibody IFN beta inducing factor antibody IL 1 antibody IL 1 beta antibody IL-1 beta antibody IL1 antibody IL1 BETA antibody IL1B antibody IL1B_HUMAN antibody IL1F2 antibody Interleukin 1 beta antibody Interleukin 1 beta precursor antibody interleukin 1, beta antibody Interleukin-1 beta antibody OAF antibody Osteoclast activating factor antibody OTTHUMP00000162031 antibody Preinterleukin 1 beta antibody Preinterleukin beta antibody Pro interleukin 1 beta antibody

Abbreviation

IL1B

Swiss Prot

P01584 Human, P10749 Mouse, Q63264 Rat

Cellular Locus

Extracellular exosome, Secreted, Lysosome, Cytosol.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human IL-1 beta aa 117-269 / 269.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, ELISA, IF-Cell

Positive Control

THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate, RAW264.7 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate, HeLa cells treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;ELISA: 1:20,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 31 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No