BRG1 Mouse Monoclonal Antibody [A5C10]

About this product

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP.

Specifications

Catalog number

HA601020

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ATP dependent helicase SMARCA4 antibody ATP-dependent helicase SMARCA4 antibody BAF 190 antibody BAF190 antibody BAF190A antibody Brahma protein like 1 antibody BRG1 antibody BRG1 associated factor 190A antibody BRG1 protein antibody BRG1-associated factor 190A antibody BRM/SWI2 related gene 1 antibody Global transcription activator homologous sequence antibody global transcription activator snf2l4 antibody Homeotic gene regulator antibody hSNF2b antibody Mitotic growth and transcription activator antibody MRD16 antibody Nuclear protein GRB1 antibody Protein brahma homolog 1 antibody Protein BRG-1 antibody Protein BRG1 antibody RTPS2 antibody SMARC A4 antibody SMARCA4 antibody SMCA4_HUMAN antibody SNF2 antibody SNF2 beta antibody SNF2 like 4 antibody SNF2-beta antibody SNF2B antibody SNF2L4 antibody SNF2LB antibody Sucrose nonfermenting like 4 antibody SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 antibody SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 antibody SWI2 antibody Transcription activator BRG1 antibody

Abbreviation

Transcription activator BRG1 ATP-dependent helicase SMARCA4 BRG1-associated factor 190A BAF190A Mitotic growth and transcription activator Protein BRG-1 Protein brahma homolog 1 SNF2-beta SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4

Swiss Prot

P51532 Human

Cellular Locus

Nucleus.

Host

Mouse

Species Reactivity

Human

Immunogen

Synthetic peptide within human BRG1 aa 240-290.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

K562 cell lysates, HeLa, human kidney tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50- 1:200 ;IF-Cell: 1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 185 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No