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Product details

HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]

Catalog number HA601037

Price for HA601037

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Size / count 100 µL

About this product

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

Specifications

Catalog number
HA601037
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
A26C1A antibody A26C1B antibody ACTB antibody ACTB_HUMAN antibody Actin beta antibody Actin cytoplasmic 1 antibody Actin, cytoplasmic 1, N-terminally processed antibody Actx antibody b actin antibody Beta cytoskeletal actin antibody Beta-actin antibody BRWS1 antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody β-actin antibody β actin antibody
Abbreviation
ACTB
Swiss Prot
P60709 Human, P60710 Mouse, P60711 Rat
Cellular Locus
Cytoplasm.
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within N-terminal human Beta-actin .
Isotype
IgG1
Conjugation
HRP-conjugated
Type
Recombinant Mouse monoclonal Antibody
Applications
WB
Positive Control
HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, THP-1 cell lysate, MCF-7 cell lysate, RH-35 cell lysate, L6 cell lysate, MEF cell lysate, HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, A549 cell lysate, PMVEC cell lysate.
Concentration
0.5 mg/mL
Dilution
WB: 1:2,500-1:20,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
Molecular Weight
Predicted band size: 42 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]?

The catalog number for HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] is HA601037.

What sizes are available for HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]?

HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] is available in the following size(s): 100 µL.

Is HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] in stock?

HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] is currently made to order / not held in immediate stock. Contact us for lead time.

What is HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] used for?

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

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