GSDMD Mouse Monoclonal Antibody [A8D8]

About this product

Gasdermin D (GSDMD) is a protein that in humans is encoded by the GSDMD gene on chromosome 8. It belongs to the gasdermin family which is conserved among vertebrates and comprises six members in humans, GSDMA, GSDMB, GSDMC, GSDMD, GSDME (DFNA5) and DFNB59 (Pejvakin). Members of the gasdermin family are expressed in a variety of cell types including epithelial cells and immune cells. GSDMA, GSDMB, GSDMC, GSDMD and GSDME have been suggested to act as tumour suppressors. Several current studies have revealed that GSDMD serves as a specific substrate of inflammatory caspases (caspase-1, -4, -5 and -11) and as an effector molecule for the lytic and highly inflammatory form of programmed cell death known as pyroptosis. Hence, GSDMD is an essential mediator of host defence against microbial infection and danger signals. The pore-forming activity of the N-terminal cleavage product causes cell swelling and lysis to prevent intracellular pathogens from replicating, and is required for the release of cytoplasmic content such as the inflammatory cytokine interleukin-1β (IL-1β) into the extracellular space to recruit and activate immune cells to the site of infection. GSDMD has an additional potential role as an antimicrobial by binding to cardiolipin (CL) and form pores on bacterial membranes. Mutation of GSDMD is associated with various genetic diseases and human cancers, including brain, breast, lung, urinary bladder, cervical, skin, oral cavity, pharynx, colon, liver, cecum, stomach, pancreatic, prostate, oesophageal, head and neck, hematologic, thyroid and uterine cancers.

Specifications

Catalog number

HA601047

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

1810036L03Rik antibody DF 5L antibody DF5L antibody DFNA 5L antibody DFNA5L antibody FKSG 10 antibody FKSG10 antibody FLJ12150 antibody Gasdermin antibody Gasdermin D antibody Gasdermin domain containing 1 antibody Gasdermin domain containing protein 1 antibody Gasdermin domain-containing protein 1 antibody Gasdermin-D antibody GasderminD antibody GSDMD antibody GSDMD_HUMAN antibody GSDMDC 1 antibody GSDMDC1 antibody

Abbreviation

GSDMD

Swiss Prot

P57764 Human

Cellular Locus

Cytosol, Inflammasome; Secreted, Cell membrane.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within human GSDMD aa 101-300/484.

Isotype

IgG2b

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB

Positive Control

PC-3 cell lysate, SiHa cell lysate, THP-1 cell lysate.

Concentration

2 mg/mL

Dilution

WB: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No