M13 Recombinant Mouse Monoclonal Antibody [PS00-01]

About this product

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 50 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 2700 copies of p8 to make the coat about 900 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single stranded genome it packages. The general stages to a viral life cycle are: infection, replication of the viral genome, assembly of new viral particles and then release of the progeny particles from the host. Filamentous phage use a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single stranded DNA genome to a double stranded replicative form.

Specifications

Catalog number

HA601052

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Bacteriophage M13 antibody Coliphage M13 antibody Enterobacteria phage M13 antibody M13 virus antibody Phage M13 antibody

Abbreviation

Bacteriophage M13 Coliphage M13 Enterobacteria phage M13 M13 virus Phage M13

Swiss Prot

P69541 BacteriophageM13

Host

Mouse

Immunogen

M13 Bacteriophage.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Recombinant Mouse monoclonal Antibody

Applications

ELISA

Positive Control

M13 Bacteriophages.

Concentration

1 mg/mL

Dilution

ELISA:1:2,500-1:20,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes