CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R]

Catalog number HA601062

Price for HA601062

Size / count 100 µL

About this product

CD133 antigen, also known as prominin-1, is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins, which specifically localize to cellular protrusions. When embedded in the cell membrane, the membrane topology of prominin-1 is such that the N-terminus extends into the extracellular space and the C-terminus resides in the intracellular compartment. The protein consists of five transmembrane segments, with the first and second segments and the third and fourth segments connected by intracellular loops while the second and third as well as fourth and fifth transmembrane segments are connected by extracellular loops. CD133 is the most commonly used marker for isolation of cancer stem cell (CSC) population from different tumors, mainly from various gliomas and carcinomas. CD133+ melanoma cells are considered a subpopulation of CSC and play a critical role in recurrence. Moreover, CD133+ melanoma cells are immunogenic and can be used as an antimelanoma vaccination. In mice the vaccination with CD133+ melanoma cells mediated strong anti-tumor activity that resulted in the eradication of parental melanoma cells. In addition, it has also been shown that CD133+ melanoma cells preferentially express the RNA helicase DDX3X . As DDX3X also is an immunogenic protein, the same anti-melanoma vaccination strategy can be employed to give therapeutic antitumor immunity in mice.

Specifications

Catalog number: HA601062
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: AC133 antibody Antigen AC133 antibody CD133 antibody CORD12 antibody Hematopoietic stem cell antigen antibody hProminin antibody MCDR2 antibody MSTP061 antibody OTTHUMP00000217744 antibody OTTHUMP00000217745 antibody OTTHUMP00000217746 antibody PROM1 antibody PROM1_HUMAN antibody Prominin I antibody Prominin like 1 antibody Prominin like protein 1 precursor antibody Prominin mouse like 1 antibody Prominin-1 antibody Prominin-like protein 1 antibody Prominin1 antibody PROML1 antibody RP41 antibody STGD4 antibody
Abbreviation: PROM1, PROML1, MSTP061
Swiss Prot: O43490 Human
Cellular Locus: Endoplasmic reticulum. Plasma membrane. Cell projection.
Host: Mouse
Species Reactivity: Human
Immunogen: Recombinant protein within human CD133 aa 151-400/865.
Isotype: IgG1
Conjugation: Non-conjugated
Type: Recombinant Mouse monoclonal Antibody
Applications: WB, IHC-P
Positive Control: Caco-2 cell lysate, HT-29 cell lysate, NCCIT cell lysate, SW480 cell lysate, human colon carcinoma tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000-1:5,000 ;IHC-P: 1:1,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 97 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R]?

The catalog number for CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R] is HA601062.

What sizes are available for CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R]?

CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R] is available in the following size(s): 100 µL.

Is CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R] in stock?

CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R] is currently made to order / not held in immediate stock. Contact us for lead time.

What is CD133 Recombinant Mouse Monoclonal Antibody [A8C3-R] used for?

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.