Myc tag Recombinant Mouse Monoclonal Antibody [A3C8-R-2]

About this product

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellulite localization studies by immunofluorescence or detection by Western blotting. The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): EQKLISEEDL and Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway. A monoclonal antibody against the myc epitope, named 9E10, is available from the non-commercial Developmental Studies Hybridoma Bank.

Specifications

Catalog number

HA601081

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

c-myc tag antibody Myc Epitope Tag antibody

Abbreviation

c-myc tag Myc Epitope Tag

Host

Mouse

Species Reactivity

Universal

Immunogen

Synthetic peptide EQKLISEEDL conjugated to keyhole limpet haemocyanin.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Recombinant Mouse monoclonal Antibody

Applications

ELISA, WB, IP, IF-Cell

Concentration

1 mg/mL

Dilution

ELISA: 1:10,000 ;WB: 1:2,000-1:100,000 ;IP: Use at an assay dependent concentration. ;IF-Cell: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes