beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]

Catalog number HA601082

Discontinued

Price for HA601082

Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

Specifications

Catalog number: HA601082
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: Yes
CAS Number: 9000-83-3
Product Name Alternative: A26C1A antibody A26C1B antibody ACTB antibody ACTB_HUMAN antibody Actin beta antibody Actin cytoplasmic 1 antibody Actin, cytoplasmic 1, N-terminally processed antibody Actx antibody b actin antibody Beta cytoskeletal actin antibody Beta-actin antibody BRWS1 antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody β-actin antibody β actin antibody
Abbreviation: ACTB
Swiss Prot: P60709 Human, P60710 Mouse, P60711 Rat
Cellular Locus: Cytoplasm.
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within N-terminal human Beta-actin .
Isotype: IgG1
Conjugation: Non-conjugated
Type: Recombinant Mouse monoclonal Antibody
Applications: WB
Positive Control: HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, MCF-7 cell lysate, THP-1 cell lysate, PMVEC cell lysate, RH-35 cell lysate, RAW264.7 cell lysate, B16F1 cell lysate, MEF cell lysate, Hela cell lysate, F9 cell lysate, Mouse lung cell lysate, L6 cell lysate.
Concentration: 1 mg/mL
Dilution: WB: 1:5,000-1:80,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 42 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]?

The catalog number for beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] is HA601082.

Is beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] in stock?

beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] is currently made to order / not held in immediate stock. Contact us for lead time.

What is beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] used for?

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.