BCL6 Recombinant Mouse Monoclonal Antibody [A6C7-R]

About this product

The Bcl-6 protein (B-cell CLL/lymphoma 6, zinc finger protein 51) is a nuclear zinc finger transcription factor with an N-terminal POZ domain. Bcl-6 represses p53 transcription by binding 2 specific DNA sites within the p53 promoter region and accordingly, p53 expression is absent in germinal centre B-cells where Bcl-6 is highly expressed. Most notably, constitutive expression of Bcl-6 protects B-cell lines from apoptosis induced by DNA damage. Bcl-6 is mainly used in the classification of B-cell lymphomas. In malignant lymphomas, Bcl-6 protein is most commonly detected in germinal centre neoplasms including follicular lymphoma, Burkitt lymphoma, diffuse large B-cell lymphoma (40%), lymphocyte predominant Hodgkin lymphoma, and even in peripheral T-cell lymphoma with follicular localization (“follicular T cell lymphoma”). It is also found in CD30+ anaplastic large cell lymphoma. Bcl-6 expression is virtually absent in acute lymphatic leukaemia and mantle cell lymphoma. Normal tonsil is recommended as positive and negative tissue control. Virtually all the germinal centre B-cells must show a moderate to strong nuclear staining reaction, while an at least weak to moderate nuclear staining reaction must be seen in the majority of squamous epithelial cells. In the mantle zones and interfollicular areas only dispersed cells should display a positive nuclear staining reaction.

Specifications

Catalog number

HA601083

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

B cell CLL/lymphoma 6 antibody B cell lymphoma 6 protein antibody B-cell lymphoma 5 protein antibody B-cell lymphoma 6 protein antibody BCL 5 antibody Bcl 6 antibody BCL-5 antibody BCL-6 antibody BCL5 antibody BCL6 antibody BCL6_HUMAN antibody BCL6A antibody cys his2 zinc finger transcription factor antibody cys-his2 zinc finger transcription factor antibody LAZ 3 antibody LAZ 3 protein antibody LAZ3 antibody Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) antibody Lymphoma associated zinc finger gene on chromosome 3 antibody Protein LAZ-3 antibody ZBTB 27 antibody ZBTB27 antibody Zinc finger and BTB domain containing protein 27 antibody Zinc finger and BTB domain-containing protein 27 (ZBTB27) antibody Zinc finger and BTB domain-containing protein 27 antibody Zinc Finger Protein 51 (ZNF51) antibody Zinc finger protein 51 antibody zinc finger transcription factor BCL6S antibody ZNF 51 antibody ZNF51 antibody

Abbreviation

BCL6

Swiss Prot

P41182 Human

Cellular Locus

Nucleus.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within Human BCL-6 aa 301-500 / 706.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Recombinant Mouse monoclonal Antibody

Applications

IHC-P, IF-Cell, mIHC

Positive Control

Human B lymphocytoma tissue, human lymph nodes tissue, Raji, Human tonsil.

Concentration

1.026 mg/mL

Dilution

IHC-P: 1:1,000 ;IF-Cell: 1:200 ;mIHC: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 79 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes