MSH2 Recombinant Mouse Monoclonal Antibody [10G3-R]

Catalog number HA601108

Price for HA601108

Size / count 100 µL

About this product

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP>ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Specifications

Catalog number: HA601108
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: BAT26 antibody COCA 1 antibody COCA1 antibody DNA mismatch repair protein Msh2 antibody FCC 1 antibody FCC1 antibody hMSH2 antibody HNPCC 1 antibody HNPCC antibody HNPCC1 antibody LCFS2 antibody MSH 2 antibody Msh2 antibody MSH2_HUMAN antibody MutS homolog 2 antibody MutS homolog 2 colon cancer nonpolyposis type 1 antibody MutS protein homolog 2 antibody
Abbreviation: hMSH2, MSH2
Swiss Prot: P43246 Human, P43247 Mouse, P54275 Rat
Cellular Locus: Nucleus, Chromosome.
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within N-terminal residues of Human MSH2.
Isotype: IgG1
Conjugation: Non-conjugated
Type: Recombinant Mouse monoclonal Antibody
Applications: WB, IF-Cell, IHC-P
Positive Control: HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Daudi, human colon cancer tissue, human appendix tissue, human breast cancer tissue, human stomach cancer tissue, rat brain tissue, mouse large intestine tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:2,000-1:5,000 ;IF-Cell: 1:200 ;IHC-P: 1:200-1:2,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 105 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for MSH2 Recombinant Mouse Monoclonal Antibody [10G3-R]?

The catalog number for MSH2 Recombinant Mouse Monoclonal Antibody [10G3-R] is HA601108.

What sizes are available for MSH2 Recombinant Mouse Monoclonal Antibody [10G3-R]?

MSH2 Recombinant Mouse Monoclonal Antibody [10G3-R] is available in the following size(s): 100 µL.

Is MSH2 Recombinant Mouse Monoclonal Antibody [10G3-R] in stock?

MSH2 Recombinant Mouse Monoclonal Antibody [10G3-R] is currently made to order / not held in immediate stock. Contact us for lead time.

What is MSH2 Recombinant Mouse Monoclonal Antibody [10G3-R] used for?

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.