CPSF73 Recombinant Rabbit Monoclonal Antibody [JE59-11]

About this product

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly (A) polymerase and other factors to bring about cleavage and poly (A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1. Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a. Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively.

Specifications

Catalog number

HA720025

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cleavage and polyadenylation specific factor 3 73kDa antibody Cleavage and polyadenylation specificity factor 73 kDa subunit antibody Cleavage and polyadenylation specificity factor subunit 3 antibody CPSF 73 antibody CPSF 73 kDa subunit antibody CPSF antibody CPSF-73 antibody cpsf3 antibody CPSF3_HUMAN antibody CPSF73 antibody mRNA 3''-end-processing endonuclease CPSF-73 antibody YSH1 antibody

Abbreviation

CPSF3

Swiss Prot

Q9UKF6 Human, Q9QXK7 Mouse

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human CPSF73 aa 585-684/684.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

293T cell lysates, rat large intestine tissue, human liver carcinoma tissue, human kidney tissue, human fallopian tube tissue, human small intestine tissue, mouse cerebellum tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

77 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes