PIAS1 Recombinant Rabbit Monoclonal Antibody [JE36-84]

About this product

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB.

Specifications

Catalog number

HA720076

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AR interacting protein antibody DDXBP1 antibody DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody DEAD/H box binding protein 1 antibody DEAD/H box-binding protein 1 antibody E3 SUMO-protein ligase PIAS1 antibody GBP antibody Gu binding protein antibody Gu-binding protein antibody GU/RH-II antibody Pias1 antibody PIAS1_HUMAN antibody Protein inhibitor of activated STAT protein 1 antibody Protein inhibitor of activated STAT, 1 antibody RNA helicase II binding protein antibody RNA helicase II-binding protein antibody Zinc finger, MIZ-type containing 3 antibody ZMIZ3 antibody

Abbreviation

PIAS1

Swiss Prot

O75925 Human, O88907 Mouse

Cellular Locus

Nucleus speckle, PML body.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within human PIAS1 aa 71-120/651.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

293T cell lysates, human thyroid tissue, human colon carcinoma tissue, mouse kidney tissue, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:500-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 72 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes