REST Recombinant Rabbit Monoclonal Antibody [JE32-25]

About this product

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 3. Repressor activity may be inhibited by forming heterodimers with isoform 3, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes. Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes. Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons.

Specifications

Catalog number

HA720081

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Neural Restrictive Silencer Factor antibody Neural-restrictive silencer factor antibody Neuron Restrictive Silencer Factor antibody NRSF antibody RE1 silencing transcription factor antibody RE1-silencing transcription factor antibody Repressor binding to the X2 box antibody rest antibody REST protein antibody REST_HUMAN antibody X2 box repressor antibody XBR antibody

Abbreviation

REST

Swiss Prot

Q13127 Human

Cellular Locus

Nucleus, Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human REST aa 850-1000.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HL-60 cell lysate, HUVEC cell lysate, human thyroid tissue, human seminoma tissue, SH-SY5Y.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IF-Cell: 1:200 ;IHC-P: 1:400

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 122 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes