iFluor™ 488 Conjugated Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]

About this product

Cytokeratin 5 is commonly found in the cells on the outermost layer of skin in humans and animals. It is encoded by the KRT5 gene and pairs with the type I keratin K14. Cytokeratin 5 has become an important biomarker for different types of cancer, including mesothelioma, breast cancer and lung cancer. It helps differentiate squamous carcinomas from adenocarcinomas. Pathologists use cytokeratin 5 to distinguish mesothelioma from adenocarcinoma, the most common type of lung cancer. Cytokeratin 5/6 cannot identify mesothelioma on its own. Cytokeratin 5/6 is a positive marker for malignant pleural mesothelioma, found in more than three-fourths of cases. It is also found in certain types of lung cancers and breast cancers. Pathologists use cytokeratin 5/6 to stain cancer tissue samples. Cytokeratin 5/6 immunoreactivity is rarely seen in adenocarcinomas of the lung. If a tumor sample shows strong expression of cytokeratin 5/6, it gives pathologists a hint the tumor is malignant mesothelioma rather than a metastatic adenocarcinoma. However, this marker is not effective for all cell types of mesothelioma. Cytokeratin 5/6 staining is usually weak or negative for sarcomatoid mesothelioma, the least common and hardest-to-treat cell type of the asbestos-related cancer. The marker is also not effective in telling the difference between pleural mesothelioma and squamous cell carcinomas. About 25 to 30 percent of all lung cancers are squamous cell carcinomas.

Specifications

Catalog number

HA720129F

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

58 kDa cytokeratin antibody CK-5 antibody CK5 antibody Cytokeratin-5 antibody Cytokeratin5 antibody DDD antibody DDD1 antibody EBS2 antibody epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody K2C5_HUMAN antibody K5 antibody keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody Keratin 5 antibody Keratin antibody keratin complex 2, basic, gene 5 antibody keratin, type II cytoskeletal 5 antibody Keratin-5 antibody Keratin5 antibody KRT 5 antibody Krt5 antibody KRT5A antibody type II cytoskeletal 5 antibody Type-II keratin Kb5 antibody

Abbreviation

CK-5, K5, KRT5

Swiss Prot

P13647 Human

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within human Cytokeratin 5 aa 70-110.

Isotype

IgG

Conjugation

iFluor™ 488

Type

Recombinant Rabbit monoclonal Antibody

Applications

IF-Tissue, IF-Cell

Positive Control

Human skin tissue, A431.

Concentration

1 mg/mL

Dilution

IF-Tissue: 1:100-1:200;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Molecular Weight

Predicted band size: 62 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes