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Product details

iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39]

Catalog number HA720156F

Price for HA720156F

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Size / count 100 µL

About this product

O-linked N-acetylglucosamine (O-GlcNAc) transferase (also designated OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related; the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.

Specifications

Catalog number
HA720156F
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
FLJ23071 antibody GlcNAc transferase antibody HRNT1 antibody MGC22921 antibody O GlcNAc antibody O GlcNAc transferase p110 subunit antibody O GlcNAc transferase subunit p110 antibody O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody O linked N acetylglucosamine (GlcNAc) transferase antibody O linked N acetylglucosamine transferase 110 kDa subunit antibody O-GlcNAc transferase subunit p110 antibody O-linked N-acetylglucosamine transferase 110 kDa subunit antibody ogt antibody OGT1_HUMAN antibody UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
Abbreviation
OGT
Swiss Prot
O15294 Human
Cellular Locus
Nucleus. Cytoplasm. Membrane.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within Human OGT aa 997-1,046 / 1,046.
Isotype
IgG
Conjugation
iFluor™ 488
Type
Recombinant Rabbit monoclonal Antibody
Applications
IF-Cell
Positive Control
Hela.
Concentration
1 mg/mL
Dilution
IF-Cell: 1:75
Purity
Protein A affinity purified.
Form
Liquid
Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Molecular Weight
Predicted band size: 117 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39]?

The catalog number for iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39] is HA720156F.

What sizes are available for iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39]?

iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39] is available in the following size(s): 100 µL.

Is iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39] in stock?

iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39] is currently made to order / not held in immediate stock. Contact us for lead time.

What is iFluor™ 488 Conjugated OGT Recombinant Rabbit Monoclonal Antibody [JB44-39] used for?

O-linked N-acetylglucosamine (O-GlcNAc) transferase (also designated OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes m

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

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