GRSF1 Recombinant Rabbit Monoclonal Antibody [JE63-10]

About this product

The protein encoded by this gene is a cellular protein that binds RNAs containing the G-rich element. The protein is localized in the cytoplasm, and has been shown to stimulate translation of viral mRNAs in vitro. Multiple transcript variants encoding different isoforms have been found for this gene. Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA.

Specifications

Catalog number

HA721014

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

G rich RNA sequence binding factor 1 antibody G rich sequence binding factor 1 antibody G-rich sequence factor 1 antibody GRSF-1 antibody GRSF1 antibody GRSF1_HUMAN antibody

Abbreviation

GRSF1

Swiss Prot

Q12849 Human, Q8C5Q4 Mouse,

Cellular Locus

Mitochondrion matrix; Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human GRSF1 aa 101-150/480.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Hela cell lysate, 293 cell lysate, Raw264.7 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat heart tissue lysate, human pancreas tissue, Daudi, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:500;IF-Cell: 1:50;IHC-P: 1:400;FC: 1:500-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes