NDUFB9 Recombinant Rabbit Monoclonal Antibody [JE63-09]

About this product

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 is an enzyme that in humans is encoded by the NDUFB9 gene. NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9 is an accessory subunit of the NADH dehydrogenase (ubiquinone) complex, located in the mitochondrial inner membrane. It is also known as Complex I and is the largest of the five complexes of the electron transport chain. The protein encoded by this gene is an accessory subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I) that is not directly involved in catalysis. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein complex has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Specifications

Catalog number

HA721015

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

B22 antibody CI B22 antibody CI-B22 antibody complex I B22 subunit antibody Complex I-B22 antibody DKFZp566O173 antibody FLJ22885 antibody I B22 antibody LYR motif containing protein 3 antibody LYR motif-containing protein 3 antibody LYRM3 antibody NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa antibody NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 antibody NADH ubiquinone oxidoreductase B22 subunit antibody NADH-ubiquinone oxidoreductase B22 subunit antibody NDUB9_HUMAN antibody Ndufb9 antibody UQOR22 antibody

Abbreviation

NDUFB9

Swiss Prot

Q9Y6M9 Human, Q9CQJ8 Mouse,

Cellular Locus

Mitochondrion inner membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human NDUFB9 aa 30-179/179.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

HepG2 cell lysate, Jurkat cell lysate, 293T cell lysate, mouse heart tissue lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat heart tissue lysate, rat kidney tissue, human liver carcinoma tissue, human stomach tissue, mouse heart tissue, PC-3M, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IF-Cell: 1:100 ;IHC-P: 1:100-1:400 ;FC: 1:500-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 22 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes