CLPX Recombinant Rabbit Monoclonal Antibody [JE62-96]

About this product

ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial is an enzyme that in humans is encoded by the CLPX gene. This protein is a member of the family of AAA Proteins (AAA+ ATPase) and is to form the protein complex of Clp protease. ClpX is an ATP-dependent chaperone that can recognize protein substrates by binding to protein degradation tags. These tags can be short unstructured peptide sequences (e.g., ssrA-tag in E coli). As an essential component of ClpP protease complex, ClpX recruits degradable substrates and unfolds their tertiary structure, which requires energy provided by ATP hydrolysis. Subsequently, these ClpX Chaperones transfer protein substrates into the proteolytic chamber formed by ClpP tetradecamer. In mammals, ClpXP protease is a pivotal contributor to mitochondrial protein quality control. A compromised ClpXP function usually leads to the accumulation of damaged proteins and mitochondrial dysfunctions, which believes to be potential causes for neurodegenerative diseases and aging.

Specifications

Catalog number

HA721016

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody AU014732 antibody Caseinolytic mitochondrial matrix peptidase chaperone subunit antibody Caseinolytic peptidase X (E.coli) antibody Caseinolytic protease X antibody clpX antibody ClpX caseinolytic peptidase X homolog antibody ClpX caseinolytic protease X homolog antibody CLPX_HUMAN antibody E330029I21 antibody Energy dependent regulator of proteolysis antibody

Abbreviation

CLPX

Swiss Prot

O76031 Human, Q9JHS4 Mouse, Q5U2U0 Rat

Cellular Locus

Mitochondrion, mitochondrion nucleoid.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human CLPX aa 584-633/633.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

Raji cell lysate, Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, Rat heart tissue lysate, Mouse heart tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue, NCI-H441.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IF-Cell: 1:50 ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 69 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes