BLNK Recombinant Rabbit Monoclonal Antibody [JE63-50]

About this product

The B-cell linker protein is encoded by the BLNK gene and is an adaptor protein also known as SLP-65, BASH, and BCA. BLNK is expressed in B cells and macrophages and plays a large role in B cell receptor signalling, in a fashion analogous to the role its paralogue SLP-76 plays in T cell receptor signalling. As it has no known intrinsic enzymatic activity, the function of BLNK is to temporally and spatially coordinate and regulate signalling effectors downstream of the B cell receptor. The function of BLNK was first illustrated in BLNK deficient DT40 cells, a chicken B-cell line, which exhibited an abrogated intracellular calcium mobilisation response and impaired activation of MAP kinases p38, JNK, and to a lesser degree ERK upon B-cell receptor (BCR) activation as compared to wild type DT40 cells. In knockout mice, BLNK deficiency results in a partial block in B-cell development, and in humans BLNK deficiency results in a much more profound block in B-cell development. Linker or adaptor proteins provide mechanisms by which receptors can amplify and regulate downstream effector proteins. The B-cell linker protein is essential for normal B-cell development.

Specifications

Catalog number

HA721028

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AGM4 antibody B cell adapter containing SH2 domain protein antibody B cell adapter containing Src homology 2 domain protein antibody B cell adaptor containing SH2 domain antibody B cell linker antibody B cell linker protein antibody B cell-specific adaptor protein antibody B-cell activation antibody B-cell adapter containing a SH2 domain protein antibody B-cell adapter containing a Src homology 2 domain protein antibody B-cell linker protein antibody BASH antibody Bca antibody BLNK antibody BLNK s antibody BLNK_HUMAN antibody Cytoplasmic adapter protein antibody Ly 57 antibody Ly57 antibody Lymphocyte antigen 57 antibody Lyw 57 antibody Lyw57 antibody MGC111051 antibody SH2 domain-containing leukocyte protein, 65-KD antibody SLP 65 antibody SLP-65 antibody SLP65 antibody Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody Src homology 2 domain containing leukocyte protein of 65 kDa antibody Src homology 2 domain-containing leukocyte protein of 65 kDa antibody

Abbreviation

BLNK

Swiss Prot

Q9QUN3 Mouse, Q4KM52 Rat, Q8WV28 Human

Cellular Locus

Cell membrane, Cytoplasm.

Host

Rabbit

Species Reactivity

Mouse, Rat, Human

Immunogen

Recombinant protein within human BLNK aa 357-456/456.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

Mouse spleen tissue lysate, rat brain tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 50 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes