BCKDK Recombinant Rabbit Monoclonal Antibody [JE63-77]

About this product

Branched chain ketoacid dehydrogenase kinase (BCKDK) is an enzyme encoded by the BCKDK gene on chromosome 16. This enzyme is part of the mitochondrial protein kinases family and it is a regulator of the valine, leucine, and isoleucine catabolic pathways. BCKDK is found in the mitochondrial matrix and the prevalence of it depends on the type of cell. Liver cells tend to have the lowest concentration of BCKDK, whereas skeletal muscle cells have the highest amount. Abnormal activity of this enzyme often leads to diseases such as maple syrup urine disease and cachexia. BCKDK regulates the activity of branched-chain α-ketoacid dehydrogenase complex (BCKD) through phosphorylation and inactivation. This inactivation results in increased branched-chain amino acids (BCAA), which is seen to reduce oxidative stress; however, having too much BCAA has been proven to be toxic to humans. Therefore, BCKDK is a vital tool to assist with BCAA homeostasis. As stated earlier, BCKDK concentrations vary depending on the type of tissue that is observed, whereas BCKD's concentration is the same in any tissue. Although BCKD concentration is constant, the amount of BCKDK determines the activity of the dehydrogenase complex. Since liver tissue is seen to have the lowest concentration of BCKDK, the activity of BCKD is seen to be the highest, delineating the fact that the BCKD kinase inversely affects the BCKD activity.

Specifications

Catalog number

HA721032

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

[3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody BCKD kinase antibody BCKD-kinase antibody BCKD_HUMAN antibody BCKDHKIN antibody Bckdk antibody BCKDKD antibody BDK antibody Branched chain alpha keto acid dehydrogenase kinase antibody Branched chain ketoacid dehydrogenase kinase antibody Branched-chain alpha-ketoacid dehydrogenase kinase antibody mitochondrial antibody

Abbreviation

BCKDK

Swiss Prot

O14874 Human

Cellular Locus

Mitochondrion matrix, Mitochondrion.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within human BCKDK aa 50-100/412.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

HAP1 cell lysate, K-562 cell lysate, Mouse kidney tissue lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes