Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92]

Catalog number HA721034

Price for HA721034

Size / count 100 µL

About this product

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Specifications

Catalog number: HA721034
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: H3 histone family 3A antibody H3 histone family 3B antibody H3 histone, family 3B (H3.3B) antibody H3.3 antibody H3.3A antibody H3.3B antibody H33_HUMAN antibody H3F3 antibody H3F3A antibody H3f3b antibody Histone H3.3 antibody Histone H3.3Q antibody Histone H3.A antibody Histone H3.B antibody MGC87782 antibody MGC87783 antibody
Abbreviation: H3-3A, H3-3B
Swiss Prot: P84243 Human, P84245 Rat
Cellular Locus: Nucleus, Chromosome.
Host: Rabbit
Species Reactivity: Human, Rat
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser31 of Human Histone H3.3.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell
Positive Control: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate, PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, HeLa cells treated with 100ng/mL Nocodazole for 18 hours.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:1,000 ;IF-Cell: 1:25,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 15 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92]?

The catalog number for Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92] is HA721034.

What sizes are available for Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92]?

Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92] is available in the following size(s): 100 µL.

Is Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92] in stock?

Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92] used for?

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.