PIAS1 Recombinant Rabbit Monoclonal Antibody [JE30-36]

About this product

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

Specifications

Catalog number

HA721035

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AR interacting protein antibody DDXBP1 antibody DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody DEAD/H box binding protein 1 antibody DEAD/H box-binding protein 1 antibody E3 SUMO-protein ligase PIAS1 antibody GBP antibody Gu binding protein antibody Gu-binding protein antibody GU/RH-II antibody Pias1 antibody PIAS1_HUMAN antibody Protein inhibitor of activated STAT protein 1 antibody Protein inhibitor of activated STAT, 1 antibody RNA helicase II binding protein antibody RNA helicase II-binding protein antibody Zinc finger, MIZ-type containing 3 antibody ZMIZ3 antibody

Abbreviation

PIAS1

Swiss Prot

O75925 Human, O88907 Mouse, G3V9T0 Rat

Cellular Locus

Nucleus, Nucleus speckle, Cytoplasm; Nucleus speckle, PML body, Nucleus; Nucleus speckle, PML body.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human PIAS1 aa 600-651.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

MCF-7 cell lysate, HL-60 cell lysate, rat testis tissue lysate, rat testis tissue, human tonsil tissue, human thyroid tissue, human colon carcinoma tissue, mouse hippocampus tissue, mouse brain tissue, NCI-H441.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IF-Cell: 1:50 ;IHC-P: 1:100-1:400

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 68 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes