PNKP Recombinant Rabbit Monoclonal Antibody [JE64-21]

About this product

Bifunctional polynucleotide phosphatase/kinase is an enzyme that in humans is encoded by the PNKP gene. A detailed structural study of the crystallized mouse protein examined both the 5´-polynucleotide kinase and 3’-polynucleotide phosphatase activities. Additional features of the peptide sequence include a forkhead association (FHA) domain, ATP binding site and nuclear and mitochondrial localization sequences. PNKP has been shown to interact with DNA polymerase beta and XRCC1. PNKP has been shown to interact with DNA polymerase beta and XRCC1. The human gene encoding PNKP was observed to be mutated in patients with microcephaly, seizures and defects in DNA repair. A type of recessive ataxia is also associated with PNKP mutations. There are also newly characterized pathological variants of PNKP. Model organisms such as mice and Drosophila have been used to generate further insights.

Specifications

Catalog number

HA721044

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

2'' (3'')-polynucleotidase antibody 2' (3')-polynucleotidase antibody Bifunctional polynucleotide phosphatase/kinase antibody DEM 1 antibody DEM1 antibody DNA 5' kinase/3' phosphatase antibody DNA 5''-kinase/3''-phosphatase antibody EIEE10 antibody Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody MCSZ antibody PNK 1 antibody PNK1 antibody Pnkp antibody PNKP DNA kinase antibody PNKP_HUMAN antibody Polynucleotide 3'-phosphatase antibody Polynucleotide 5' hydroxyl kinase antibody Polynucleotide 5''-hydroxyl-kinase antibody Polynucleotide kinase 3 prime phosphatase antibody Polynucleotide kinase 3' phosphatase antibody Polynucleotide Kinase antibody Polynucleotide kinase-3''-phosphatase antibody

Abbreviation

PNKP

Swiss Prot

Q96T60 Human

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human PNKP aa 51-100 / 521.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

Jurkat cell lysate, HepG2 cell lysate, PC-3 cell lysate, Hela cell lysate, human breast carcinoma tissue, human kidney tissue, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:500-1,000;IF-Cell: 1:100;IHC-P: 1:400

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 57 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes