TPMT Recombinant Rabbit Monoclonal Antibody [JE64-09]

About this product

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT) is an enzyme that in humans is encoded by the TPMT gene. A pseudogene for this locus is located on chromosome 18q. Thiopurine methyltransferase methylates thiopurine compounds. The methyl donor is S-adenosyl-L-methionine, which is converted to S-adenosyl-L-homocysteine. This enzyme metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents and immunosuppressive drugs. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. About 1/300 individual is deficient for the enzyme. Genetic variants of TPMT have also been associated with cisplatin-induced ototoxicity in children. TPMT is now listed as a pharmacogenomic biomarker for adverse drug reactions to cisplatin by the FDA.

Specifications

Catalog number

HA721049

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

HGNC:12014 antibody S adenosyl L methionine thiopurine S methyltransferase antibody Thiopurine methyltransferase antibody Thiopurine S methyltransferase antibody Thiopurine S-methyltransferase antibody TPMT antibody TPMT_HUMAN antibody

Abbreviation

TPMT

Swiss Prot

P51580 Human, O55060 Mouse, Q9Z0T0 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human TPMT aa 101-150/245.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

K562 cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, A431 cell lysate, mouse brain tissue lysate, rat stomach tissue lysate, mouse kidney tissue lysate, rat lung tissue lysate, mouse hippocampus tissue, K562, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IHC-P: 1:400-1:1,000 ;FC: 1:500-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 28 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes