ACAT-1 Recombinant Rabbit Monoclonal Antibody [JE64-40]

About this product

Acetyl-CoA acetyltransferase, mitochondrial, also known as acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT1 (Acetyl-Coenzyme A acetyltransferase 1) gene. Acetyl-Coenzyme A acetyltransferase 1 is an acetyl-CoA C-acetyltransferase enzyme. This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The ACAT1 enzyme has a few unique properties. First, it is activated by potassium ions binding near the CoA binding site and the catalytic site. This binding causes a structural change in the active site loop. Additionally, this enzyme is able to use 2-methyl-branched acetoacetyl-CoA as a substrate, making it a unique thiolase. ACAT1 is regulated at both transcriptional and translational levels. ACAT1 enzyme activity is enhanced ACAT1’s expression is promoted transcriptionally by leptin, angiotensin II, and insulin in human monocytes/macrophages. Insulin-mediated regulation also involves ERK, p38MAPK, and JNK signaling pathways.

Specifications

Catalog number

HA721093

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ACAT 1 antibody ACAT antibody acat1 antibody Acetoacetyl CoA thiolase antibody acetoacetyl Coenzyme A thiolase antibody Acetoacetyl-CoA thiolase antibody Acetyl CoA acetyltransferase, mitochondrial antibody Acetyl Coenzyme A acetyltransferase 1 antibody Acetyl-CoA acetyltransferase antibody acetyl-coa acetyltransferase precursor, mitochondrial antibody Acetyl-CoA thiolase, mitochondrial antibody acetyl-Coenzyme A acetyltransferase 1 antibody MAT antibody mitochondrial acetoacetyl-CoA thiolase antibody mitochondrial antibody RATACAL antibody T2 antibody testicular tissue protein Li 198 antibody THIL antibody THIL_HUMAN antibody

Abbreviation

ACAT1

Swiss Prot

P24752 Human, Q8QZT1 Mouse, P17764 Rat

Cellular Locus

Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human ACAT1 aa 378-427/427.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

Raji cell lysate, HepG2 cell lysate, rat liver tissue lysate, rat heart tissue lysate, HepG2, human liver tissue, human liver carcinoma tissue, rat liver tissue, mouse liver tissue, mouse heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:100-1:400 ;IF-Cell: 1:250

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 45 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes