NDUFB4 Recombinant Rabbit Monoclonal Antibody [JE65-30]

About this product

NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa is a protein that in humans is encoded by the NDUFB4 gene. The NDUFB4 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. The human NDUFB4 gene codes for a subunit of Complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. However, NDUFB4 is an accessory subunit of the complex that is believed not to be involved in catalysis. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein complex has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Specifications

Catalog number

HA721112

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

B15 antibody CI-B15 antibody Complex I B15 subunit antibody Complex I-B15 antibody NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 15kDa antibody NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 antibody NADH ubiquinone oxidoreductase B15 subunit antibody NADH-ubiquinone oxidoreductase B15 subunit antibody NDUB4_HUMAN antibody NDUFB4 antibody

Abbreviation

NDUFB4

Swiss Prot

O95168 Human, Q9CQC7 Mouse,

Cellular Locus

Mitochondrion inner membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human NDUFB4 aa 1-129/129.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

Mouse heart tissue lysates, rat heart tissue lysate, rat kidney tissue lysate, rat brain tissue lysate, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IHC-P: 1:400

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes