CD5 Recombinant Rabbit Monoclonal Antibody [PD00-01]

About this product

The CD5 antigen is a 67 kDa transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10% of CD4+ T-cells being CD5 negative). In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increaing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20% of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone). The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthrititis and systemic lupus erythematosus. CD5 is detected in most T-cell lymphomas and leukaemias, including 75% of peripheral T-cell lymphomas and 85% of T-ALL cases. Lack of CD5 in the latter signifies a worse prognosis. Among B-cell lymphomas, more explicit CD20+ small-cell lymphomas, small lymphocytic lymphoma and mantle cell lymphoma are CD5+, whereas follicular lymphoma, marginal zone lymphoma and lymphoplasmacytoid lymphoma are CD5 negative. CD5 is detected in 5% of acute myeloid leukaemias. CD5 has been detected in some cases of thymic carcinoma and atypical thymoma. Other carcinomas are CD5 negative. Classification of small B-cell lymphomas, prognostication of T-ALL. Identification of large cell lymphoma (CD5 negative) supervening a CD5+ small lymphocytic lymphoma. Differentiation between reactive CD5+ T-cell infiltration and CD5 negative T-cell neoplasm. Identification of thymic carcinoma. Tonsil is recommendable as positive and negative tissue control, in which dispersed B-cells in the mantle zone of the secondary follicles must display a weak to moderate and distinct membranous staining reaction. T-cells will be strongly stained. No staining must be seen in the germinal center B-cells.

Specifications

Catalog number

HA721137

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CD 5 antibody CD5 antibody CD5 antigen (p56 62) antibody CD5 antigen antibody CD5 molecule antibody CD5_HUMAN antibody LEU1 antibody LyA antibody Lymphocyte antigen T1/Leu-1 antibody OTTHUMP00000236973 antibody T-cell surface glycoprotein CD5 antibody T1 antibody

Abbreviation

CD5

Swiss Prot

P06127 Human

Cellular Locus

Cell membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human CD5 aa 403 – 495 (intracellular).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IHC-P

Positive Control

Human lymph nodes tissue, human spleen tissue.

Concentration

1 mg/mL

Dilution

IHC-P: 1:1,500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 55 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes