CD20 Recombinant Rabbit Monoclonal Antibody [PD00-02]

About this product

The CD20 antigen is a membrane-embedded, non-glycosylated phosphoprotein, 33-37 kDa. CD20 functions as a Ca2+-permeable cation channel, involved in the regulation of B-cell activation, proliferation and differentiation. CD20 appears on the surface of the pre-B lymphocyte between the time of light chain rearrangement and expression of intact surface immunoglobulin and is lost just before terminal B-cell differentiation into plasma cells. CD20 is virtually specific for normal B-cells. A weak expression has been demonstrated in a subpopulation of T-cells, but not in any other cell type. CD20 is expressed in the large majority of cases of B-cell leukaemia/lymphoma. Early stage precursor B lymphoblastic leukaemia/lymphoma may be negative, and chronic lymphocytic leukaemia/small cell lymphoma may show a weak staining. Plasma cell neoplasms are as a rule CD20 negative. T-cell lymphomas are almost always negative, but CD20 has been demonstrated in few cases of various types of T-cell lymphoma. In Hodgkin lymphoma, the nodular lymphocyte-predominant subtype shows CD20 staining of L&H cells in most cases, while Reed-Sternberg cells in the other subtypes reveal CD20 positivity in about 40, albeit in a minority of neoplastic cells. Acute myeloid leukaemia is CD20 positive in few cases, while blastic transformation in chronic myeloid leukaemia is accompanied by CD20 positivity in about 30%. Thymoma may reveal CD20 positivity in a spindle cell component. In patients treated with retuximab (a humanized anti-CD20 antibody) for malignant B-cell lymphoma, the CD20 epitopes disappear (both in normal and neoplastic B-cells) as a result of down-modulation of CD20 m-RNA in the cells. This process is potentially reversible. Together with CD79a, CD20 is one of the most important markers for the identification of B-cell neoplasms as outlined above. Tonsil and appendix are appropriate controls: The mantle zone B-cells and the germinal centre B-cells must show a very strong staining reaction. No other cells should stain.

Specifications

Catalog number

HA721138

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

APY antibody ATOPY antibody B lymphocyte antigen CD20 antibody B Lymphocyte Cell Surface Antigen B1 antibody B-lymphocyte antigen CD20 antibody B-lymphocyte cell-surface antigen B1 antibody B-lymphocyte surface antigen B1 antibody B1 antibody Bp 35 antibody Bp35 antibody CD 20 antibody CD20 antibody CD20 antigen antibody CD20 receptor antibody CD20_HUMAN antibody CVID 5 antibody CVID5 antibody Fc epsilon receptor I beta chain antibody Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody FCER1B antibody High affinity immunoglobulin epsilon receptor subunit beta antibody IgE Fc receptor subunit beta antibody IGEL antibody IGER antibody IGHER antibody LEU 16 antibody Leu-16 antibody LEU16 antibody leukocyte surface antigen Leu 16 antibody Leukocyte surface antigen Leu-16 antibody Ly44 antibody Membrane spanning 4 domains A1 antibody Membrane spanning 4 domains subfamily A member 2 antibody membrane-spanning 4-domains A1 antibody Membrane-spanning 4-domains subfamily A member 1 antibody MGC3969 antibody MS4A1 antibody MS4A2 antibody S7 antibody

Abbreviation

MS4A1

Swiss Prot

P11836 Human, P19437 Mouse

Cellular Locus

Cell membrane.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human CD20 aa 210-297 (Cytoplasmic).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IHC-P, mIHC, WB, IF-Cell, IP

Positive Control

Human tonsil tissue, human lymph nodes tissue, human spleen tissue, human small cell lung cancer, human non-small cell lung cancer, human prostate cancer, human non-small cell lung cancer, mouse spleen tissue, Raji cell lysate, Ramos cell lysate, Daudi cell lysate.

Concentration

1 mg/mL

Dilution

IHC-P: 1:1,000-1:2,000 ;mIHC: 1:1,500-1:3,000 ;WB: 1:2,000 ;IF-Cell: 1:500 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 33 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes